TNF alpha antibody

Catalog No. ABIN217729

This Mouse Monoclonal antibody specifically detects TNF alpha in ELISA and FACS. It exhibits reactivity toward Cow.

Quick Overview for TNF alpha antibody (ABIN217729)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Product Details anti-TNF alpha Antibody

Specificity: Recognizes bovine TNF alpha, a 17.5kD cytokine, expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes. The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN-beta, NGF, Oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumor and toxic effects and under certain conditions is also capable of self-stimulation and inhibition. This reagent may be used as a capture antibody in ELISA assays for bovine TNF alpha in combination with MCA2335B as detection reagent.

Purification: Protein G purified

Immunogen: Recombinant bovine TNF alpha.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, Flo

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Membrane permeabilization is required for this application. ELISA: This reagent may be used as a capture antibody in sandwich ELISA assays for bovine and sheep TNF alpha in combination with LS-C44702 as detection reagent.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Background: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Gene ID: 7124

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome